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Spirulinasp.is an alga that has antioxidant and anti-inflammatory potential.Phycocyanin contained in Spirulina platensis can reduce tumor necrosis factor-? levels, neutrophils, and M2 macrophages in inflammation. This research aims to investigate the anti-inflammatory activity of S. platensisin a mice wound model. Thirty-two male Wistar rats were incised and divided into 4 groups. The first therapeutic group (X1) received S. platensisextract at a dose of 500 mg/kgBW/day, and the second therapeutic group received S. platensisextract at a dose of 750 mg/kgBW/day. The negative control group (C1) received a saline solution and the positive control group (C2) received diclofenac sodium 20mg/kgBW/day. Serum TNF-? levels examined by the Enzyme-Linked Immunosorbent Assaymethod. Neutrophils and M2 macrophages were calculated by tissue biopsy and hematoxylin-eosin (HE) staining. Data analysis was performed with ANOVA test and LSD Post-Hoc Test. The average levels of serum TNF-? on the 14-day were 394.50; 180.33; 2,980.33; and 607.42 pg/ml for X1, X2, C1 and C2, respectively. The average neutrophils number on the 14-day were 8.00; 6.83; 14.67; and 11.17 for X1, X2, C1 and C2, respectively. The average macrophage number in the 14-day was 15.50; 19.17; 6.33; and 11.17 for X1, X2, C1 and C2, respectively. We found significant differences between TNF-? levels, the number of neutrophils, and the number of M2 macrophages. The administration of S. platensisextract at a dose of 750 mg/ kgBW/day reduces serum TNF-? levels, neutrophil count, and increases M2 macrophages in skin incisional mice model.